Alterations in oxidative metabolism in liver of female rats: Effects of long-term vitamin A deficiency
Department of Biochemistry and Biological Sciences, School of Chemistry, Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 13(01), 267-278.
Article DOI: 10.30574/gscbps.2020.13.1.0064
Publication history:
Received on 12 March 2020; revised on 15 October 2020; accepted on 20 October 2020
Abstract:
Background: The latest estimate by 5 UN agencies is that 821 million people globally are undernourished, which puts them at risk of vitamin and other nutrient deficiencies. Vitamin A deficiency remains a widespread public health problem among women and children in the developing world the role of vitamin A and its active metabolites in pathways involved in antioxidant protection and in the inhibition of important pathways that promote oxidative stress.
Objectives: Determine if vitamin A deficiency could influence oxidative metabolism in 6-month-old female wistar rats.
Materials and Methods: Determine the concentration of carbonyl proteins, as a marker of protein oxidation; TBARS, as a lipoperoxidation marker; and nitrotyrosine as a marker of oxidative stress dependent on nitric oxide. Quantify the expression of CAT, SOD, eNOS and iNOS in the liver and wistar rats deficient in vitamin A for 6 months.
Results: An increase in the concentration of carbonyl protein and nitrotyrosine in the liver tissue deficient in vitamin A is observed. The expression of SOD, eNOS and iNOS decreased in the group with a private diet of vitamin A. From the regression analysis a positive correlation was established between hepatic retinoic acid levels and gene expression of eNOS, iNOS and SOD. A positive correlation between serum retinoic acid levels and gene expression of eNOS and iNOS was also observed.
Conclusions: It is possible to ratify the relationship between the development of stress and vitamin A levels; improving the understanding of hepatic metabolism and its response to the absence of this vitamin.
Keywords:
Lipoperoxidation; Nitrotyrosine; Catalase; Superoxide dismutase; Nitric oxide synthetase
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