Antibiotics resistance and phenotypic virulence factors of Vibrio species isolated from poultry litters at Obafemi Awolowo University, research farm.
1 Department of Microbiology and Biotechnology, First Technical University, Ibadan, Oyo, Nigeria.
2 Department of Nutrition and Health Sciences, Universidade Federal de Vicosa, Brazil.
3 Department of Veterinary Medicine, Laboratory of Bacterial Diseases, (LDBAC) Universidade Federal de Viçosa, Minas Gerais, Brazil.
4 Institute of Ecology and Environmental Studies, Faculty of Science, Obafemi Awolowo University, Ile-Ife Nigeria.
5Department of Microbiology, University of Ilorin, Kwara State, Nigeria.
6Department of Animal Sciences, University of Ibadan, Oyo, Nigeria.
7Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria.
8 Genetics and Molecular Biology Laboratory, Department of Biology, College of Science and Technology, Texas Southern University, USA.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 27(01), 028–034.
Article DOI: 10.30574/gscbps.2024.27.1.0102
Publication history:
Received on 16 February 2024; revised on 31 March 2024; accepted on 02 April 2024
Abstract:
The study investigates the antimicrobial resistance and phenotypic virulence factors of Vibrio isolates in poultry litter. Samples were enriched in 5 mL alkaline peptone water 1:10 (w/v) for 6 hours and cultured on Thiosulphate Citrate Bile salt (TCBS) agar. Vibrio isolates were identified through biochemical tests assay and were subjected to antibiotic susceptibility tests using standards disc diffusion technique. The phenotypic virulence factors, including hemolytic and proteolytic activity were determined. A total of 100 Vibrio isolates were identified as Vibrio parahaemolyticus (49%), V. cholerae (26%), V. vulnificus (11%), V. alginolyticus (9%), and V. damsela (5%). The Vibrio isolates showed a significant resistance to the selected antibiotics tested with 99% resistant to Gentamycin, 95% Cefixime, 96% Ofloxacin, 98% Augmentin, 96% Nitrofurantoin, 100% Ciprofloxacin, and 100% Cefuroxime. The Vibrio isolates showed varying levels of haemolysis with 17% alpha haemolysis, 61% beta haemolysis, 22% gamma haemolysis, and 29% proteolytic activity. The study reveals that poultry litter contains Vibrio isolates with antibiotic resistance and virulence factors, increasing their risk of severe infections. The high beta haemolysis prevalence highlights the interconnected relationship between animal, environmental, and human health, underscoring the need for effective management.
Keywords:
Antibiotics resistance; Pathogenicity; Poultry liters; Vibrio; Virulence factors.
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
