Effect of different doses of nitrogen on nitrogen fixation and yield of lentil using tracer technique
1 Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, G.P.O. Box No-3787, Dhaka-1000, Bangladesh.
2 Soil Science Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.
3 Agriculture Research Section, Philippine Nuclear Research Institute, Commonwealth Ave., Diliman, Quezon City 1101, Phillipine.
Research Article
GSC Biological and Pharmaceutical Sciences, 2019, 06(03), 069–075.
Article DOI: 10.30574/gscbps.2019.6.3.0027
Publication history:
Received on 20 February 2019; revised on 11 March 2019; accepted on 15 March 2019
Abstract:
Legumes play a predominant role in nitrogen cycling having the unique features of biological nitrogen fixation. An experiment was conducted to estimate yield, nitrogen use efficiency (NUE ) and the proportion of nitrogen fixation in plant segments i.e. roots, shoots, leafs and seeds of lentil. Three treatments such as 0 kg/ha, 11.5 kg/ha and 25.3 kg/ha were used in this experiment. The highest dry matter yield obtained were 43.44 kg/ha, 2677 kg/ha, 1450 kg/ha and 1604 kg/ha at roots, shoots, leaves and seeds respectively with the treatment 11.5 kg/ha N level. 15N labelled urea was applied to wheat and lentil at 5% 15N atom excess. The total nitrogen content in lentil was found 8.39 % with an average yield of 104.5 kg N/ha whereas it was 1.95% with an average yield of 102.8 kg N/ha in case of wheat. Nitrogen derived from fertilizer in lentil was 1.173% with an average fertilizer N yield of 12.085 kg/ha whereas it was 7.538% with an average yields of 223.16 kg N/ha in wheat. Lentil fixed around 85% of its required nitrogen from atmosphere through biological fixation and a minimal amount of 14.387% of nitrogen was drawn from soil source. Nitrogen use efficiency in seed was 110.1 % in lentil and 36.22% in wheat which were much higher than the other plant segments in both cases.
Keywords:
Lentil; 15N isotope; 5% Atom excess; NUE; Labeled urea
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Copyright © 2019 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
