Improvement of protocol for detachment assays in B16F10 cells
Department of biochemistry institute of chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Sao Paulo, 05508-900, Brazil.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 22(01), 211–213.
Article DOI: 10.30574/gscbps.2024.27.1.0132
Publication history:
Received on 05 March 2024; revised on 13 April 2024; accepted on 16 April 2024
Abstract:
B16F10 (B16) murine melanoma cells have the characteristic of strong adhesion to culture surfaces. The use of trypsin solutions in high doses to release this type of cell is not suitable. When B16F10 cells are released with high concentrations of trypsin they do not form a precipitate after centrifugation and consequently the cells will be lost when discarding the supernatant. To solve this problem, we established a solution with phosphate buffered saline (PBS) and ethylenediaminetetraacetic acid (EDTA) at low trypsin concentrations. Finally, we verified the efficiency of this solution on the quantity of cells recovered after centrifugation.
Keywords:
Improvement of protocol; B16F10 melanoma cells; Detachment assays; Trypsin enzyme; PBS/EDTA assays
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
